TB Green Advantage qPCR Premix-Taq Master Mix with Hot Start &TB GreenTB Green Advantage qPCR Premix is a convenient, ready-to-use 2X-concentrated master mix that contains full-length Taq polymerase with hot start antibody andTB Green, and is specially designed for real-time PCR employing the dye intercalator method. This product combines the high performance of our enzyme for hot start PCR utilizing Taq antibody and a newly developed buffer, to provide superior specificity, increased amplification efficiency, and excellent performance in high-speed, real-time PCR. Use of theTB GreenAdvantage qPCR Premix enables you to carry out successful real-time PCR with high sensitivity, broad dynamic range, and accurate quantification. The reagent is supplied separately with ROX Reference Dye LSR and ROX Reference Dye LMP, which allow you to normalize the fluorescent signal between reactions on instruments that are equipped with this option. Recent studies have shown that the ClontechTB GreenAdvantage qPCR Premix is superior to corresponding reagents offered by three leading competitors in the qPCR arena, in terms of reaction specificity, amplification efficiency, and sensitivity.More Specific than Competitor RCompetitor R’s real-time enzyme showed poor reaction specificity when compared to the ClontechTB GreenAdvantage qPCR Premix. This was evidenced by multiple peaks in the melting curve for Competitor R"s enzyme, particularly when low-copy-number templates were amplified (Figure 1).More Efficient than Competitor ACompetitor A"sTB Greenshowed a lower amplification efficiency than that of the ClontechTB GreenAdvantage qPCR Premix. This was indicated by Ct values that were shifted to the right (Figure 2).More Specific than Competitor IThe ClontechTB GreenAdvantage qPCR Premix demonstrated superior reaction specificity when compared to Competitor I"s mix. This was indicated by tighter peaks in the melting curve for the Clontech enzyme (Figure 3).Twice as Fast as Competitor AExcellent results were obtained in about half the time (50 min versus 90 min) when using the ClontechTB GreenAdvantage qPCR Premix as opposed to Competitor A"s TaqMan mix (data not shown). These experiments were carried out with the Applied Biosystems 7500 Real-Time PCR System. Clearly, the ClontechTB GreenAdvantage qPCR Premix outperforms much of the competition in terms of specificity (melting curves indicate the presence of a single product), efficiency, and sensitivity (lower Ct values in the amplification plots). Moreover, when using the ClontechTB GreenAdvantage qPCR Premix, qPCR analysis can be accomplished in nearly half of the time required for other qPCR chemistries.

For GC-Rich TargetsUseTB GreenAdvantage GC qPCR Premix to amplify difficult, GC-rich targets. This 2X master mix offers the same advantages and benefits as theTB GreenAdvantage qPCR Premix, but it’s optimized for targets with a GC content between 60-70%.Compatible cyclers:SmartCycler, LightCycler, ABI PRISM 7000/7700/7900 HT, Applied Biosystems 7300/7500, iCycler, Opticon, Stratagene MX 3000P, and Rotor-Gene.

□ Components

TB GreenAdvantage qPCR Premix2XTB GreenAdvantage qPCR Premix50X ROX Reference Dye LSR50X ROX Reference Dye LMPTB GreenAdvantage GC qPCR Premix2XTB GreenAdvantage GC qPCR Premix50X ROX Reference Dye LSR50X ROX Reference Dye LMP

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□ Storage Conditions

● Store at -70℃ in a dark container

● After thawing, store at 4℃ in a dark container - Do not refreeze!