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abfrontier/EpiXplore Meth-Seq DNA富集试剂盒/EpiXplore Meth-Seq DNA富集试剂盒，每个/635023
abfrontier/EpiXplore Meth-Seq DNA富集试剂盒/EpiXplore Meth-Seq DNA富集试剂盒，每个/635023
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产品分类：
ELISA辅助试剂
公司分类：
ELISA_helper_reagent
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
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제품특징
□ 특징
● Rapid, user-friendly protocol which separates methylated DNA from unmethylated DNA
● Ligation-independent sequencing adapter addition for library preparation
● DNA inputs of 25 ng?1 μg sheared genomic DNA
● Generate Illumina®-ready sequencing libraries from the enriched DNA in ~4 hour
□ 제품설명
EpiXplore™ Meth-SeqDNA Enrichment Kit은 sheared genomic DNA로부터 메틸화된 DNA와 비메틸화된 DNA 절편을 분리하고 그로부터 Illumina® sequencing library를 제작 가능한 실험에 필요한 모든 구성품이 포함된 complete system이다. EpiXplore™ Meth-SeqDNA Enrichment Kit를 이용하면 Clontech 독자의 CapturemNanoprep column 기술과 His-taggedMBD2 protein을 조합한 DNA Enrichment기술을 이용하여 메틸화 및 비메틸화 DNA 분리를 2시간 이내에 진행할 수 있다 (그림 1). 이후, DNASMART® 기술을 이용하여 메틸화 DNA 절편 (또는, 비메틸화 DNA 절편)으로부터 Illumina® compatible sequencing library를 제작한다 (그림 2). NGS Library Prep은 ligation-independent DNA SMART® 방법으로 DNA 절편의양 말단에 Illumina®-specific sequencing adaptor를 부가한다. 본 방법을 이용하면 low-input DNA 샘플로부터 4시간 내에 Sequencing library를 제작 할 수 있다.
그림 1. Flowchart for separatingmethylated and unmethylated fractions of DNA. In the enrichment portion of the protocol, sheared DNA is incubated withhis-tagged MBD2 protein for 1 hr, which allows the methylated DNA fraction tobind to the protein. The solution is then added to the Capturem nanoprepcolumn, which binds the his-tagged protein and thus the methylated DNA.Subsequent washes with the Unmethylated Elution Buffer remove the unmethylatedDNA. The methylated DNA is then eluted in a high-salt buffer. After cleanup,this enriched DNA can be used as the input for library preparation.
그림 2. Flowchart for DNA-SMART ChIP-Seq technology. This single-tube workflow allows users to generateIllumina-compatible libraries for meth-seq experiments. After library sizeselection and purification, the total time from input DNA to meth-seq libraryis approximately four hours. DNA SMART technology eliminates the need for anadapter ligation step and associated cleanup, reducing the loss of limitedinput DNA.
그림 3. Distinct methylation patterns are observed in aligned NGS dataof libraries generated from methylated and unmethylated DNA fractions. A representative region of Arabidopsis chromosome 3(outlined in the green box) shows the peaks identified in the DNA enriched formethylated and unmethylated fractions. The methylated regions are outlined inred boxes and the unmethylated regions are outlined in blue boxes. The amountof initial input DNA is shown along the left-hand side of the alignments. Thedata show good concordance with the publicly available bisulfite sequencingdata. Data were visualized using the Integrative Genomics Viewer (BroadInstitute). Public data was obtained from GEO accession GSM980986; Stroud etal., 2013.
□ Application
- Sequencing library preparation for methylated DNA-seq fromlow-input, sheared genomic DNA - Illumina®-specific NGS sequencing library production
□ 구성품
- EpiXplore Meth-Seq DNAEnrichment Components - SeqAmp™ DNA Polymerase - DNA SMART ChIP-Seq Components - Indexing Primer Set HT forIllumina - 12品牌介绍
Abfrontier是Young In Frontier的开创性抗体品牌，是抗胰腺抗体技术的领先供应商。 Abfrontier最初的举措主要集中在活性氧（ROS）途径产品，但是Abfrontier正在进行的研发活动集中在Abfrontier的产品线上，扩展到神经科学，血清蛋白和特异性抗体进行翻译后修饰。 此外，Abfrontier已经生产了世界上第一批氧化特异性抗体系列，这表明了Abfrontier的创新和专业。Abfrontier还能提供一系列的服务，包括蛋白质组分析，DNA克隆，蛋白质纯化和抗体生产和纯化。自2004 年以来，为全球客户提供超过30,000种定制抗体--HuPO的主要抗体合作伙伴（Human Protein Atlas） 拥有4,000个动物笼的大型设施
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