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当前位置: 首页 > 产品中心 > Voltage_potential > abfrontier/ThruPLEX标签序列套件/ThruPLEX标签序列48S套件,48회/红蓝4584
商品详细abfrontier/ThruPLEX标签序列套件/ThruPLEX标签序列48S套件,48회/红蓝4584
abfrontier/ThruPLEX标签序列套件/ThruPLEX标签序列48S套件,48회/红蓝4584
abfrontier/ThruPLEX标签序列套件/ThruPLEX标签序列48S套件,48회/红蓝4584
商品编号: RB4584
品牌: adipoGen
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
公司:
产品分类: 电压电位
公司分类: Voltage_potential
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍

주문정보

견적신청주문하기장바구니관심상품
  • - 재고수량은 변동될 수 있습니다.
  • - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
  • - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
선택Cat.No.제품명가격(VAT별도)수량
RB4584ThruPLEX Tag-seq 6S(12)Kit, 12회로그인후 확인가능 합니다.
RB4586ThruPLEX Tag-seq 96D Kit, 96회로그인후 확인가능 합니다.
RB4585ThruPLEXTag-seq 48S Kit, 48회로그인후 확인가능 합니다.

제품특징

□ 특징

● Results you can trust.ThruPLEX® Tag-seq technology provides up to 16million molecular tags to correct sequencing errors providing confident variantdetection.

● Discover more, costeffectively. Use with hybridization-based targetenrichment systems to examine hundreds of genes, including mutations andstructural variants, simultaneously.

● High quality libraries thefirst time, every time.Unparalleled ease of use reduces user error and contamination with oursingle-tube, 2-hour, 3-step workflow.

● Time-saving bioinformaticssolutions. Reach results quickly with cloud-basedsoftware from Curio Genomics or open-source scripts available for dataprocessing.

● Precious samples go further.Use less DNA to analyze samples once too low to detect. ThruPLEX® Tag-seq workswith input amounts from 1 ng to 50 ng of cfDNA or fragmented dsDNA toaccommodate a wide range of samples.

□ 제품설명

ThruPLEX® Tag-seq Kit은 moleculartag와 ThruPLEX chemistry를 결합시켜, 샘플 index가 부가된 IlluminaNGS library를 제작할 수 있는 제품이다. ThruPLEX® Tag-seq Kit의 Stem-Loop adaptor는 1600만 개의 unique molecular tag(UMT)을 포함하고있어서, PCR 증폭 전에각각의 DNA 단편에 UMT을 부가 할 수 있다. 그 결과, NGS 데이터 분석에서 위양성 비율을 감소시켜 보다 정확한 결과를 얻을 수 있다.

□ 보관

Store at -20 °C.Guarantee for 9 months at -20°C in a constant temperature freezer.

□ 내용

Component name

Cap

Color

6S Kit 6 Single Indexes

48S Kit48 Single Indexes

96D Kit96 Dual Indexes

Code

 

RB4584

RB4585

RB4586

용량

 

12

48

96

TemplatePreparation Buffer

Red

1 Tube

1 Tube

2 Tubes

TemplatePreparation Enzyme

Red

1 Tube

1 Tube

2 Tubes

LibrarySynthesis Buffer

Yellow

1 Tube

1 Tube

2 Tubes

LibrarySynthesis Enzyme

Yellow

1 Tube

1 Tube

2 Tubes

LibraryAmplification Buffer

Green

1 Tube

1 Tube

2 Tubes

LibraryAmplification Enzyme

Green

1 Tube

1 Tube

2 Tubes

Nuclease-FreeWater

Clear

1 Tube

1 Tube

1 Tube

IndexingReagents

Blue

6 Tubes

1 Single IndexPlate (48S)

1 Dual IndexPlate (96D)

※ ThruPLEX® Tag-seq Kit single Index 서열 * ThruPLEX Tag-seq 6S Kit (RB4584) * ThruPLEX Tag-seq 48S Kit (RB4585)[그림1] Single-Tube, Three step workflow helps your precious samples gofurther Startingwith 1 to 50 ng of DNA, ThruPLEX® Tag-seq Kit creates indexedlibraries in 3 simple steps: end repair, adapter ligation,and high-fidelitylibrary amplification. No purification or sample transfer steps are required.The streamlined workflow is performedin 2 hours in a single tube or well, preventingsample loss and enhancing positive sample identification. [그림 2] ThruPLEX® Technology generates high quality librariesthe first time, every timeThruPLEX® technologyis a 3-step reaction that starts with fragmented double-stranded DNA orcell-free DNA which is repaired in a highly efficient process. Background isreduced using double-stranded adaptors with no single-stranded tails. Blunt endligation occurs with high-efficiency. Blocked 5" ends reduce adapter-adapterligation.[그림 3] Reduce background forresults you can trust ThruPLEX® Tag-seqlibraries were prepared from 30 ng of the 1% Horizon cfDNA standard. FollowingAgilent SureSelect target enrichmentand sequencing to achieve ~10,000Xcoverage, data was analyzed with the Curio Genomics bioinformatics platform.Using the raw reads,the EGFR mutation (yellow dot) is obscured by backgrounderrors (left). The mutation is readily identified when UMTs are utilized in data processing to correct for errors (right).[그림 4] Dramatically increase the signal to noise ratio to discover moreData from either the 30 ngHorizon cfDNA library(A) or a ThruPLEX® Tag-seq library preparedfrom 10 ng Horizon cfDNA standard, enriched with a custom 240 KB panel(NimbleGen SeqCapEZ) and sequenced on a HiSeq 2500 to achieve ~5,000X coverage.Analysis was performed using the Curio Genomics bioinformatics platform.

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品牌介绍
Abfrontier是Young In Frontier的开创性抗体品牌,是抗胰腺抗体技术的领先供应商。 Abfrontier最初的举措主要集中在活性氧(ROS)途径产品,但是Abfrontier正在进行的研发活动集中在Abfrontier的产品线上,扩展到神经科学,血清蛋白和特异性抗体进行翻译后修饰。 此外,Abfrontier已经生产了世界上第一批氧化特异性抗体系列,这表明了Abfrontier的创新和专业。Abfrontier还能提供一系列的服务,包括蛋白质组分析,DNA克隆,蛋白质纯化和抗体生产和纯化。自2004 年以来,为全球客户提供超过30,000种定制抗体--HuPO的主要抗体合作伙伴(Human Protein Atlas) 拥有4,000个动物笼的大型设施