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abfrontier/In-Fusion Ready Products/pDsRed-Monomer-N In-Fusion Ready Vector，1μg/632500
주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
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제품특징
□ pAcGFP1 Vector Information
□ Restriction map and multiple cloning site (MCS) of pAcGFP1.
Unique restriction sites are in bold. NOTE: The Xba I and Bcl I sites are methylated in the DNA provided by Clontech Laboratories, Inc. If you wish to digest the vector with these enzymes, you will need to transform the vector into a dam -host and make fresh DNA.
□ Location of features
● lac promoter: 95 - 178 CAP binding site: 111 - 124 - 35 region: 143 - 148;- 10 region: 167 - 172 Transcription start point: 179 lac operator: 179 - 199
● lacZ - ECFP fusion protein expressed in E. coli Ribosome binding site: 206 - 209 Start codon (ATG): 217 - 219; stop codon: 1006 - 1008
● 5" multiple cloning site: 234 - 281
● Aequorea coerulescens Green fluorescent protein (AcGFP1) gene Kozak consensus translation initiation site: 282 - 292 Start codon (ATG): 289 - 291; stop codon: 1006 - 1008 Insertion of Val at position 2: 292 - 294 Last amino acid: 1003 - 1005
●3" Multiple Cloning Site: 1009 - 1108Ampicillin resistance gene: Promoter:- 35 region: 1484 - 1450,- 10 region: 1507 - 1512 Ribosome binding site: 1542 - 1546 β-lactamase coding sequences:start codon (ATG): 1554 - 1556; stop codon: 2412 - 2414 β-lactamase signal peptide: 1554 - 1622 β-lactamase mature protein: 1623 - 2411
