주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
□ 특징
● His-tagged protein의 빠르고 간편하게 분리
● His60 Ni과 magnetic bead를 이용하여 고순도의 단백질 정제
● 적은 부피에서 elution
His60 Ni Magnetic Beads 는 Clontech의 His60 Ni Superflow Resin과 magnetic bead의 분리장점을 결합한 제품이다.
Bead의 자성 입자는 magnetic separator를 이용해 미소규모의 단백질을 빠르고 쉽게 분리하도록 한다.
□ Highly Specific Binding & Elution
His60 Ni Magnetic Beads는 높은 특이성으로 작은 분자량에서 큰 분자량까지 다양한 크기의 his-tagged protein과 결합한다. 정제된 단백질은 소량의 용액 (50-200 μl)으로 용출가능하여 농축된 형태로 결과가 나타난다 (~3 mg/ml). His60 Ni Magnetic Beads는 5% 현탁액으로 제공되며, ml당 1.65 mg 의 단백질과 결합할 수 있다.
□ Microscale Screening
His60 Ni Magnetic Beads는 microscale 정제는 발현 수준을 선별하거나 protein-protein interaction 연구를 위해 사용될 수 있다. 또한 His60 Ni 기술이 접목된 Clontech의 표준 His60 Ni Superflow Resin을 사용함으로써 목적 단백질 정제의 균일한 scale-up이 가능하도록 한다.
□ Applications
His60 Ni Magnetic Beads provide simple, effective separation of recombinant his-tagged proteins for a wide range of applications, including:
● Microscale purification of his-tagged proteins
● Studying protein structure and function
● Preparing proteins for x-ray crystallography
● Performing assays that detect protein-protein and protein-DNA interactions
● Immunization to raise antibodies against a protein of interest
● Screening for protein expression
● Optimizing purification conditions for scale-up with His60 Ni Resin
His60 Ni Magnetic Beads can also be used to immobilize 6xHis-tagged proteins for affinity chromatography, in order to:
● Analyze protein-protein and protein-nucleic acid interactions
● Purify untagged subunits or nucleic acids that interact with the immobilized protein
● Perform antibody purification
● Study interactions between ligands and receptors
그림1. Purifying his-tagged AcGFP1 from a cell lysate using His60 NiMagnetic Beads under native conditions. 400 μl of clarified lysate from E.coli cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads andallowed to bind for 30 minutes. The beads were washed 3X with 500 μl of WashBuffer, and eluted 4X with 100 μl of Elution Buffer (from the His60 Ni BufferSet, Cat. No. 635655). A 10 μl aliquot of each sample was analyzed using a 12%SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane 1.Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane 5.Wash 3. Lane 6. Eluate 1. Lane 7. Eluate 2. Lane 8. Eluate 3. Lane 9. Eluate 4.
그림 2. Purifying his-tagged AcGFP1 from a cell lysate using His60 NiMagnetic Beads under denaturing conditions. 500 μl of clarified lysate from E.coli cells expressing AcGFP1-6xHis was added to His60 Ni Magnetic Beads andallowed to bind for 30 minutes. The beads were washed 3X with 500 μl ofEquilibration Buffer and 1X with 500 μl of Wash Buffer-and eluted 3X with 50 μlpf Elution Buffer (from the His60 Ni Buffer Set, Cat. No. 635655). 8M urea wasadded to all the buffers. A 10 μl aliquot of each sample was analyzed using a4-20% SDS-PAGE gel. Lane M: Spectra Multicolor Broad Range Protein Ladder. Lane1. Original sample. Lane 2. Flowthrough. Lane 3. Wash 1. Lane 4. Wash 2. Lane5. Wash 3. Lane 6. Wash 4. Lane 7. Eluate 1. Lane 8. Eluate 2. Lane 9. Eluate3.
