3G塔特拉环线上的abfrontier/Tet발현 유도 시스템/3G诱导表达系统上的Tet，每个/631188
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| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
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제품특징
□ Tet-On Inducible Expression - The 3rd Generation
The Tet-On 3G Tetracycline Inducible Gene Expression Systems are the 3rd generation of the most powerful, versatile, and widely cited inducible mammalian expression systems available. The 3G system offers a significant improvement over Tet-On and Tet-On Advanced by further reducing basal expression and increasing sensitivity to doxycycline, a tetracycline analogue (Table I, Figures 1-3).
□ Tet-On 3G: Lowest Background Ever, Highest Sensitivity, Highest Fold Induction
Lowest BackgroundMutations within thePTRE3G promoter have reduced background expression from this inducible promoter by 5-20 fold compared to our previous tightest promoter PTight (Figure 1).Highest SensitivityMutations to create the Tet-On 3G transactivator have significantly increased its sensitivity to the inducer doxycycline (Dox), so that at very low Dox concentrations (between 5-10 ng/ml), expression can be 100-150 fold higher when using a Tet-On 3G system, compared to Tet-On Advanced (Figure 3). An overnight exposure to as little as 10 ng/ml of Dox generates very high expression levels (Figure 4).Highest Fold InductionFold induction refers to the difference between the induced and uninduced states. Since Tet-On 3G retains the high maximal expression demonstrated by all Tet-On and Tet-Off systems but has significantly reduced basal expression compared to its predecessors, the fold induction levels are far higher (Figure 2).We have observed unprecedented fold induction levels even in transient cotransfection experiments-up to a 27,000 fold difference between the induced and uninduced expression levels in MCF7 cells (not shown). To learn about the mechanism of tetracycline-controlled inducible expression and the changes made to both the transactivator and TRE promoter creating the Tet-On 3G Inducible Expression System, visit our Tet-On 3G resource site or read the inducible expression technical note.
Figure 1. The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On Advanced Inducible Expression Systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G System produces far lower background expression in the absence of Dox (inset).
Figure 1. The PTRE3G promoter results in significantly reduced basal expression. HEK 293 cells were transiently cotranstected with both the response vectors (containing luciferase) and regulator vectors from each of the Tet-On 3G and Tet-On Advanced Inducible Expression Systems. The cells were cultured in the presence and absence of Dox, and after 24 hr, luciferase expression was measured. Although both systems provided strong expression in the presence of Dox, the Tet-On 3G System produces far lower background expression in the absence of Dox (inset). 品牌介绍
Abfrontier是Young In Frontier的开创性抗体品牌，是抗胰腺抗体技术的领先供应商。 Abfrontier最初的举措主要集中在活性氧（ROS）途径产品，但是Abfrontier正在进行的研发活动集中在Abfrontier的产品线上，扩展到神经科学，血清蛋白和特异性抗体进行翻译后修饰。 此外，Abfrontier已经生产了世界上第一批氧化特异性抗体系列，这表明了Abfrontier的创新和专业。Abfrontier还能提供一系列的服务，包括蛋白质组分析，DNA克隆，蛋白质纯化和抗体生产和纯化。自2004 年以来，为全球客户提供超过30,000种定制抗体--HuPO的主要抗体合作伙伴（Human Protein Atlas） 拥有4,000个动物笼的大型设施
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