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abfrontier/Cell Capture，Cell Separation&Cell Enricuring-CherryPicker系统/Lenti-X CherryPicker细胞捕获系统，60 rxns/632571
abfrontier/Cell Capture，Cell Separation&Cell Enricuring-CherryPicker系统/Lenti-X CherryPicker细胞捕获系统，60 rxns/632571
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产品分类：
金属浴
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Metal_bath
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3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
□ Capture only those cells with promoter activity
Use the promoterless CherryPicker systems (CherryPicker Cell Capture System and Lenti-X™ CherryPicker Cell Capture System) to monitor the activation of a promoter of interest, and capture those cells containing the active promoter. Insertion of a functional promoter into the promoterless reporter vector, pCherryPicker1, causes a red fluorescent protein (CherryPicker) to be displayed on the surface of mammalian cells, which are then easily captured by a CherryPicker-specific antibody bound to IgG-coated magnetic beads.
□ Capture only those cells expressing your protein
Use the bicistronic CherryPicker systems [CherryPicker Cell Capture System (IRES) and Lenti-X CherryPicker Cell Capture System (IRES)] to identify, monitor, and capture mammalian cells expressing your protein of interest. IRES technology results in simultaneous expression of a protein of interest and a membrane-targeted red fluorescent protein (CherryPicker) from the same transcript. Cells expressing your protein of interest must also express CherryPicker, and can be captured on magnetic beads via a CherryPicker-specific antibody. Coexpression of your protein of interest and CherryPicker allows you to easily:1.Monitor cells expressing the protein of interest 2.Capture and analyze cells expressing the protein of interest 3.Culture the captured cells as a homogeneous population
Figure 1. Select cells for promoter activity or protein expression, using the CherryPicker Systems. Panel A. Use the promoterless systems to capture cells with promoter activity. Inserting your promoter of interest into the promoterless reporter vector causes a red fluorescent protein (CherryPicker)to be displayed on the surface of mammalian cells, which are then easily captured on magnetic beads via a CherryPicker-specific antibody.Panel B. Use the bicistronic (IRES) systems to capture cells expressing your protein of interest. Insert the gene for your protein of interestinto the IRES reporter vector. The protein will be expressed simultaneously with a membrane-targeted red fluorescent protein (CherryPicker)from the same transcript. Cells expressing your protein of interest can then be captured on magnetic beads via a CherryPicker-specific antibody. 
Figure 2. Highly efficient enrichment of CherryPicker cells from a mixed population. Two populations of cells were created, one expressing AcGFP1 and the other expressing CherryPicker. The two populations were mixed in a ratio of > 6:1 AcGFP1:CherryPicker cells. The mixed populationwas then enriched for CherryPicker-expressing cells using the CherryPicker Assay Kit. After enrichment, the ratio was > 1:4 AcGFP1:CherryPicker cells.The distribution of cells before and after capture was determined by flow cytometry.
Figure 3. Easily select cells expressing a protein of interest using the Bicistronic (IRES) CherrryPicker Systems. HEK-293 cells were transiently transfected with a pCherryPicker2-MEK-CA construct. A HEK-293 cell population containing 10% transfected cells was divided into two equal fractions. One fraction of cells was lysed without any further treatment (middle lanes). The second fraction of cells was enriched for MEK-CA expressing cells using the CherryPicker magnetic bead system prior to lysis (right lane). Both lysates were analyzed by western blot using the anti-phospho-specificErk1/2 antibody. Lane1: Lysate of 35 x103 non-transfected HEK293 cells. Lanes 2-4: Lysates equivalent of 17.5, 35, and 52.5 x103 cells, from the fraction that was lysed without enrichment. Lane 5: Lysate of captured cells from the fraction that was enriched using the magnetic bead-based CherryPicker kit. To allow for normalization, the western blot was also tested using an antibody against the ubiquitously expressed GAPDH.
Figure 1. Select cells for promoter activity or protein expression, using the CherryPicker Systems. Panel A. Use the promoterless systems to capture cells with promoter activity. Inserting your promoter of interest into the promoterless reporter vector causes a red fluorescent protein (CherryPicker)to be displayed on the surface of mammalian cells, which are then easily captured on magnetic beads via a CherryPicker-specific antibody.Panel B. Use the bicistronic (IRES) systems to capture cells expressing your protein of interest. Insert the gene for your protein of interestinto the IRES reporter vector. The protein will be expressed simultaneously with a membrane-targeted red fluorescent protein (CherryPicker)from the same transcript. Cells expressing your protein of interest can then be captured on magnetic beads via a CherryPicker-specific antibody. Figure 2. Highly efficient enrichment of CherryPicker cells from a mixed population. Two populations of cells were created, one expressing AcGFP1 and the other expressing CherryPicker. The two populations were mixed in a ratio of > 6:1 AcGFP1:CherryPicker cells. The mixed populationwas then enriched for CherryPicker-expressing cells using the CherryPicker Assay Kit. After enrichment, the ratio was > 1:4 AcGFP1:CherryPicker cells.The distribution of cells before and after capture was determined by flow cytometry.Figure 3. Easily select cells expressing a protein of interest using the Bicistronic (IRES) CherrryPicker Systems. HEK-293 cells were transiently transfected with a pCherryPicker2-MEK-CA construct. A HEK-293 cell population containing 10% transfected cells was divided into two equal fractions. One fraction of cells was lysed without any further treatment (middle lanes). The second fraction of cells was enriched for MEK-CA expressing cells using the CherryPicker magnetic bead system prior to lysis (right lane). Both lysates were analyzed by western blot using the anti-phospho-specificErk1/2 antibody. Lane1: Lysate of 35 x103 non-transfected HEK293 cells. Lanes 2-4: Lysates equivalent of 17.5, 35, and 52.5 x103 cells, from the fraction that was lysed without enrichment. Lane 5: Lysate of captured cells from the fraction that was enriched using the magnetic bead-based CherryPicker kit. To allow for normalization, the western blot was also tested using an antibody against the ubiquitously expressed GAPDH.
□ Applications
Promoterless Systems: - Monitor the activation of a promoter of interest - Capture and analyze cells in which a promoter of interest is active- Culture the captured cells as a more homogeneous populationBicistronic (IRES) Systems:- Monitor cells expressing a protein of interest - Capture and analyze cells expressing the protein of interest - Culture the captured cells as a more homogeneous population
□ Components
CherryPicker Assay KitCherryPicker Antibody Wash Buffer IgG-Coated Magnetic Capture BeadsCherryPicker Cell Capture SystempCherryPicker1 and pCherryPicker Control VectorsCherryPicker Assay KitCherryPicker Cell Capture System (IRES) pCherryPicker2 and pCherryPicker Control VectorsCherryPicker Assay KitLenti-X CherryPicker Cell Capture SystempLVX-CherryPicker1 and pLVX-CherryPicker Control VectorsCherryPicker Assay KitLenti-X CherryPicker Cell Capture System (IRES)pLVX-CherryPicker2 and pLVX-CherryPicker Control VectorsCherryPicker Assay Kit
□ Storage Conditions
CherryPicker Vectors & Antibody: -20°C Wash Buffer & Magnetic Capture Beads: 4°C
品牌介绍
Abfrontier是Young In Frontier的开创性抗体品牌，是抗胰腺抗体技术的领先供应商。 Abfrontier最初的举措主要集中在活性氧（ROS）途径产品，但是Abfrontier正在进行的研发活动集中在Abfrontier的产品线上，扩展到神经科学，血清蛋白和特异性抗体进行翻译后修饰。 此外，Abfrontier已经生产了世界上第一批氧化特异性抗体系列，这表明了Abfrontier的创新和专业。Abfrontier还能提供一系列的服务，包括蛋白质组分析，DNA克隆，蛋白质纯化和抗体生产和纯化。自2004 年以来，为全球客户提供超过30,000种定制抗体--HuPO的主要抗体合作伙伴（Human Protein Atlas） 拥有4,000个动物笼的大型设施
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