주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 수량 |
|---|
제품특징
□ Cell Cycle Monitoring Vectors (Fucci)
● Easily and precisely track cell-cycle progression in live cells
● Monitor phase transitions in cultured cells or live tissue
● Visualize cell shape during phase transitions
● Label whole cells or just nuclei
● Monitor stably expressed phase reporters over long time periods
Clontech’s cell cycle reporter vectors let you monitor cell cycle progression in living cells, in real-time, without fixation.
□ Fucci Probes
Our cell cycle reporter vectors deliver fluorescent, ubiquitination-based, cell-cycle indicators (Fucci; 1, 2) that allow you to identify cells in various phases of the cell cycle. These Fucci probes contain Cdt1 or Geminin, proteins whose levels fluctuate differentially throughout the cell cycle: Cdt1 levels peak in G1 phase; as cells transition into S phase, Cdt1 levels fall and Geminin levels rise, remaining high until the cells are back in G1. Cells control Cdt1 and Geminin levels post-translationally, using ubiquitination to target the unwanted proteins for proteasomal degradation. To allow Geminin and Cdt1 to be easily visualized, each is expressed with a red or cyan fluorescent tag. These probes allow precise, visual evaluation of the cell cycle phase (Figure 1).
□ Monitor Cell Cycle and Shape in Real-Time
Our Fucci cell cycle reporters let you label just the nucleus, or both the nucleus and cytoplasm-allowing visualization of cell shape. Although most Fucci probes label only cell nuclei, we offer a truncated version of hGeminin that is able to migrate to the cytoplasm between S and M phases, enabling the morphology of the cell to be visualized (Table I; 3).
□ A Variety of Fucci Probes and Delivery Options
We offer retrovirus- and plasmid-based vectors for the delivery of a variety of Fucci probes (see Table I). Each probe consists of a red or cyan fluorescent tag, and a deletion mutant of either hCdt1 or hGeminin. Our retroviral vectors let you express Fucci probes in a wide variety of mammalian cells. Once integrated into the host genome, these vectors provide stable, heritable expression of the probes. 
The plasmid-based vector, pTRE-CellCycle (Figure 3), allows tightly controlled, tetracycline (Tet)-inducible expression of two Fucci probes from a bidirectional promoter that can be induced using any Tet-On or Tet-Off technology. Expression of these probes causes cell nuclei to turn red during G1 phase and cyan during S, G2, and M phases, allowing complete visual tracking of the cell cycle. For more information on the latest Tet System technologies, visit the Tet-On 3G Product Page. Use Clontech’s Fucci vectors to monitor cell cycle progression in live cells, as well as cell cycle phase distributions in a cell population. Our Fucci vectors even let you monitor cell shape as the cells transition through the cell cycle.
Figure 2. Retroviral delivery is available for a variety of Fucci probes. Fucci probes containing Geminin-fluorescent protein (fp) fusions are visible in phases S through M, whereas the probe containing Cdt1-fp (expressed by pRetroX-G1-Red) is visible during the G1 phase. pRetroX-SG2Mcyto-Red expresses a truncated version of geminin that allows the cell shape to be visualized from phases S through M.
