주문정보
- - 재고수량은 변동될 수 있습니다.
- - 재고 확인 시 "갱신" 버튼을 누르시면 실시간 재고를 확인하실 수 있습니다.
- - 가격이 ‘별도문의‘ 시, 상단 ‘견적신청’ 버튼을 눌러 문의해주시면 빠른 답변을 받으실 수 있습니다.
| 선택 | Cat.No. | 제품명 | 가격(VAT별도) | 재고 | 재고 | 수량 |
|---|
제품특징
□ 제품 설명
SMART-Seq® v4 Ultra® Low InputRNA Kit for the Fluidigm® C1™ SystemOurlatest single-cell RNA-seq kit is the SMART-Seq v4 Ultra Low RNA Kit for theFluidigm C1 System, IFCs. This kit allows high-quality cDNA synthesis fromIFC-captured cells (96-IFC format) processed with the Fluidigm C1 Single-Cell AutoPrep IFC for mRNA Seq or Open App. Its method improves upon the previousgenerations by incorporating the novel use of locked nucleic acid (LNA)technology into an optimized template-switching oligo (our proven SMARTtechnology), and other advancements developed by Clontech scientists. The newversion contains SeqAmp polymerase, which excels even in GC- and AT-richregions and promotes better transcript representation.SMART-Seq v4 kits, including the SMART-Seq v4 Ultra Low RNA Kit for theFluidigm C1 System, IFCs, produce single-cell mRNA-seq libraries that clearlyoutperform previously-published protocols and older products. SMART-Seq v4 kitsprovide higher sensitivity and reproducibility (meaning more genes areidentified from libraries produced with these kits) and exhibit significantlylower background than the other methods.
□ 특징
- High-quality single-cell RNA-seq data - whole transcriptome full-length mRNA-seq information, better representation of GC-rich genes, and good reproducibility with reduced technical variation.
- Library construction in 2 days - hands-on aspects of the cDNA synthesis protocol can be completed in approximately two hours, followed by running the eight-hour SMART-Seq v4 script on the C1 instrument. The entire library construction protocol can be completed within two working days.
- New script available online - the script for using the SMART-Seq v4 Ultra Low RNA Kit for the Fluidigm C1 System is available in the Fluidigm Script Hub. The user manual for this product is available on the Documents tab of this web page.
Interestedin more data and FAQs about this product? Visit the NGS Resource Portal.
□ Applications
- cDNA synthesis for mRNA-seq from up to 96 single cells on the Fluidigm C1 System
- cDNA synthesis for qPCR analysis on the Fluidigm Biomark platform prior to Illumina sequencing
□ 구성품
- SMART-Seq®v4 Ultra® Low Input RNA Kit for the Fluidigm® C1™ System, 4 IFCs Components
- SeqAmp™ DNA Polymerase
